绿色荧光蛋白质 /Green Fluorescent Protein (GFP)
chem-station.com
绿色荧光蛋白质 /Green Fluorescent Protein (GFP)
绿色荧光蛋白(Green Fluorescent Protein;GFP)、是从维多利亚多管发光水母(Aequorea victoria)中分离出来的一种荧光蛋白。 用途・历史 诺奖得主・下村脩等人在1962年从维多利亚多管发光水母中分离・精制后获得的。[1] (照片：引用自Wikipedia) 分子量约为27 kDa、由238 ...
Jun 17, 2018
GFP Purification Protocol
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10: Green Fluorescence Protein (GFP) Purification
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libretexts.org
6 months ago
A weeks worth of hard work, mentally AND physically, was all worth it! I got so much pure protein of a novel mutation I’ve made to my protein of interest as part of my PhD project! 👩‍🔬 My protein purification protocol is very long and detailed with one of the last steps involving FPLC. My pure protein gets collected in different tubes in a fraction collector. Based on the chromatograph, I decide what fractions to pull and run on gel. I pool together the samples that have the purest samples of
0:09
A weeks worth of hard work, mentally AND physically, was all worth it! I got so much pure protein of a novel mutation I’ve made to my protein of interest as part of my PhD project! 👩‍🔬 My protein purification protocol is very long and detailed with one of the last steps involving FPLC. My pure protein gets collected in different tubes in a fraction collector. Based on the chromatograph, I decide what fractions to pull and run on gel. I pool together the samples that have the purest samples of
TikTokmaddyb.phd
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