Escherichia coli remains at the forefront of recombinant protein production, owing to its rapid growth, well-characterised genetics and the ease with which it can be genetically manipulated. The ...

Immobilized metal affinity chromatography (IMAC) is a reliable method for isolation of recombinant protein following over-expression in Escherichia coli. The polyhistidine tag (6–10 consecutive amino ...

Designing a high-density fed-batch, E. coli-based strategy to produce recombinant GLP-1 cuts upstream production costs 20–30%.

Stay up to date on the latest science with Brush Up Summaries. Finally, although transgenic animals and mammalian cell culture systems promote optimal expression due to correct protein modifications, ...