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WEB3 days ago · The biotech bi-weekly: organoids take to the stars, SPR increases in sensitivity and 3D printing brings tunability to oral drug delivery. Explore BioTechniques. A world leader in life science method research news. ISSN (print): 0736-6205 | …
Western Blotting Summary - BioTechniques
WEBWestern blotting (or immunoblotting) is a well-established biochemical technique that detects specific proteins in complex samples and has applications from basic research to the diagnosis of infectious diseases. Western blotting can be used to determine quantity, molecular weight and post-translational modifications of proteins and can ...
How Early Life Thrived Without Oxygen - BioTechniques
WEBJan 11, 2017 · According to Planavsky, oxygen saturated the surface of oceans and the atmosphere, but these sulfur-oxidizing bacteria thrived best at the interface between oxic and oxygen-poor waters. The evolution of the environment changed the course of evolution for most life on Earth. “A lot of people don’t think about how the Earth has changed so ...
Cellular Pathway for Linked Drug Molecule Uptake - BioTechniques
WEBDec 16, 2022 · To utilize what they’d discovered about the cellular uptake of large drugs, the researchers then went on to design two large, linked drugs: DasatiLink-1 and BisRoc-1. DasatiLink-1 was composed of two linked known inhibitors of BCL-ABL1, a leukemia protein. Whereas BisRoc-1 was composed of two linked chemotherapy molecules found in …
What is organ-on-a-chip technology? - BioTechniques
WEBJun 7, 2023 · How do human cells form organ and tissue replicas in vitro that can be interconnected to facilitate organ crosstalk? How does the organ-on-a-chip (OOC) technology deliver nutrients, oxygen and biomechanical cues to cells to mimic the body? Find out in this video, which introduces OOC technology and why it is an important preclinical tool. Animation […]
Protecting the powerhouse of the cell - BioTechniques
WEBJan 11, 2019 · The mitochondria, often labeled the powerhouse of the cell, are the organelle responsible for energy production within the cell. Playing an important role in cellular respiration, the mitochondria are the main location for ATP production. They also make proteins essential for DNA metabolism such as the iron-sulfur family of proteins.
Determining Drivers of Extracellular Protein Degradation
WEBNov 2, 2023 · Targeted protein degradation (TPD) is a promising therapeutic strategy that repurposes machinery within the cell to target faulty proteins for destruction. One such technology, lysosome-targeting chimeras (LYTACs), can precisely mark destructive extracellular proteins or the cell-membrane proteins …
An Overview of Mass Spectrometry - BioTechniques
WEBDec 9, 2021 · Using mass spectrometry in bioanalysis with GSK’s Scott Summerfield. 9 Dec 2021. Analytical chemistry Lab design and machinery. Scott Summerfield, head of bioanalysis at GSK, gives an overview of the steps in mass spectrometry, which includes ionization, mass selection and, finally, detection of molecules in …
T2T-CHM13 – the complete human genome sequence
WEBJun 11, 2021 · The new sequence – named T2T-CHM13 – was made possible through combining the advantages of new, competing long-read technologies from Pacific Biosciences (CA, USA) and Oxford Nanopore (UK), which increased the length of DNA accurately scannable at one time from a few hundred base pairs to 20,000. The longer pieces are – like a child’s ...
New Improvements to TIRF Microscopy - BioTechniques
WEBOct 5, 2018 · Researchers at the National Institute of Biomedical Imaging and Bioengineering (MD, USA) have combined two different microscope technologies to create sharper images of rapidly moving processes inside a cell. The recent research describes new improvements to traditional total internal reflection fluorescence (TIRF) microscopy.